Analysis Note

One mg will inhibit ≥0.8 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein.

Application

Trypsin inhibitor from Phaseolus limensis (lima bean) has been used:in the inhibition of trypsin in human plasmain the termination of proteolytic digestion in rabbit skeletal musclesin the inhibition of salivary glands enzyme extract from Lygus lineolarisin the inhibition of proteolytic activity in Agave tequilana enzyme extract

Trypsin inhibitor has been used as an affinity ligand for isolation of elastase-type enzymes.

Biochem/physiol Actions

Trypsin inhibitor from lima beans belongs to Bowman-Birk family of protease inhibitors. It is 9 kDa in molecular weight and contains nine disulfide bridges and is highly thermostable. It has trypsin and chymotrypsin binding subdomains.

Other Notes

View more information on Trypsin Inhibitor.

Packaging

1 g in glass bottle

100, 500 mg in glass bottle

Physical properties

Monomer has an apparent molecular weight of approx. 9,000 Da but undergoes a concentration and pH-dependent dimerization.

Preparation Note

Prepared by a modification of the method of Tauber, H., et al., J. Biol. Chem., 179, 1155 (1949) (modified).

Unit Definition

One trypsin unit will produce a δA₂₅₃ of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 mL, 1 cm light path.