Application

Trizma^® base has been used:as a component of H buffer (cell dissociation buffer)for washing and saturation of wells in double sandwich ELISA immunoenzymatic techniqueas an assay buffer for reconstitution of extracted and dried protein samplesto prepare Tris-HCl buffer that is used to stabilize proteinsas a buffer to extract carotenoid from tubersas a component of sample buffer during protein extraction prior to western blottingas a component of sample buffer for sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)to prepare simulated body fluid (SBF) for calcium phosphate (CaP) resorption assayas a buffer for polydopamine (PDA) deposition on stainless steel (SS) substrate

General description

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

40% (w/w) solution in water is clear and colorless.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Other Notes

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Easily compare specifications for Trizma products with the Trizma specification table.

Packaging

1 kg in poly bottle

5, 10, 25, 50 kg in poly drum

25, 100, 250, 500 g in poly bottle