Biochem/physiol Actions

CXCL11 (C-X-C motif chemokine 11) is involved in the wound repair in skin cells. CXCL11’;s interaction with CXCR3 (C-X-C chemokine receptor type 3) polarizes naive T cells into IL (interleukin)-10^hi Tregs (regulatory T cells, Tr1) and repolarizes CXCR3⁺CD4⁺ EAE (experimental autoimmune encephalomyelitis)-associated effector T cells into IL-10^hi Tregs. CXCL11 also enhances Th2 (T helper 2) response in model leishmaniasis system, and thereby regulates the outcome of the immunological pathway. In mice model, CXCL11 is upregulated in RSV (respiratory syncytial virus) and influenza A infections.

General description

CXCL11 (C-X-C motif chemokine 11), also referred to as I-TAC (interferon-inducible T-cell α chemoattractant), is from to the CXC chemokine family and is an antagonist for CC chemokine receptor 3 (CCR3) and CCR5. CXCL11 was first identified in astrocytes via cDNA sequencing. It can be induced by IFN (interferon)-γ in different cells. CXCL11 interacts with and signals via CXCR3 (C-X-C chemokine receptor type 3). Recombinant murine I-TAC is a 9.0kDa protein containing 79 amino acid residues including the four highly conserved cysteine residues present in CXC chemokines.

Physical form

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Sequence

FLMFKQGRCL CIGPGMKAVK MAEIEKASVI YPSNGCDKVE VIVTMKAHKR QRCLDPRSKQ ARLIMQAIEK KNFLRRQNM