Biochem/physiol Actions

Twisted gastrulation protein (TSG) can inhibit bone morphogenetic protein (BMP) activity by binding directly to BMP proteins, and can act either as a BMP4 agonist or antagonist (depending on the specific biochemical environment) by binding to the BMP4/chordin complex.

General description

Twisted gastrulation protein (TSG) is a secreted bone morphogenetic protein (BMP)-binding protein structurally related to the BMP antagonists chordin and noggin. The gene encoding TSG is localized on human chromosome 18p11.22. Recombinant human TSG is a 199 amino acid 22.2kDa protein containing the BMP/TGFb binding portion of the full length TSG protein.

Physical form

Lyophilized from 10 mM Sodium Acetate, pH 6.0.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Sequence

MCNKALCASD VSKCLIQELC QCRPGEGNCS CCKECMLCLG ALWDECCDCV GMCNPRNYSD TPPTSKSTVE ELHEPIPSLF RALTEGDTQL NWNIVSFPVA EELSHHENLV SFLETVNQPH HQNVSVPSNN VHAPYSSDKE HMCTVVYFDD CMSIHQCKIS CESMGASKYR WFHNACCECI GPECIDYGSK TVKCMNCMF