Application

Useful for the study of biochemical function.

General description

Human Peroxiredoxin I (GenBank Accession No. NM_005614), the full length protein containing a Lysine to Glutamine mutation at position 197 and N-terminal His6 tag, MW = 23 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.