Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Biochem/physiol Actions

PARP6 (poly(ADP-ribose) polymerase family member 6) negatively controls cell cycle, as its ectopic expression in HeLa cells leads to suppression of cell growth. It functions as a tumor suppressor in CRC (colorectal cancer), and its expression is associated with good prognosis. The ectopic expression of PARP6 results in suppressed expression of the oncogene survivin. In CRC PAPR6 prevents colony formation, invasion, migration, and cell proliferation.

General description

PARP6 (poly(ADP-ribose) polymerase family member 6) gene is localized to human chromosome 15q23. It is a member of the family of multifunctional nuclear proteases known as PARPs, which contains 17 members and is further classified into three subtypes dependent on the catalytic domain structure. PARP6 is a type of mono(ADP-ribosyl) transferase on the basis that its catalytic domain has a significantly shorter nicotinamide-ribose-binding site than PARP1. This subfamily contains nine members including PARP6. PARP6 is composed of 630 residues and has a molecular weight of 71kDa.Human PARP6 (GenBank Accession No. NM_020214), full length with N-terminal GST tag, MW = 98kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Unit Definition

One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute (room temperature) from NAD into acid-insoluble form.