Application

Useful for the western blot analysis.

General description

Human RbAp48 (NM_005610) (amino acids 2-end) with N-terminal His tag, MW = 48 kDa, expressed in baculovirus expression system.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20, 100 µg/ml flag peptide and 10% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.