Application

ATPase activity assay for Hsp90alpha.

General description

Human recombinant full-length AHA1 with C-terminal HIS tag (GenBank Accession No. NM_012111), expressed in E. coli. MW = 38 kDa,

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.