Application

HDAC-5 full-length human has been used in the adenosine diphosphate (ADP)-Glo assay and Γ-p³²-adenosine triphosphate (ATP) radioisotope assay with focal adhesion kinase (FAK) kinase.

Biochem/physiol Actions

Histone deacetylase 5 (HDAC5) mediates synoviocyte activation, neural repair and regeneration. It regulates the assembly of the pericentric heterochromatin. Elevated expression of HDAC5 is implicated in high-risk medulloblastoma and breast cancer in very young women (BCVY).

General description

Histone deacetylase 5 (HDAC5) belongs to the Hda1-like proteins class IIa HDAC family. It contains 17 phospho-acceptor residues and a classical deacetylase domain. The HDAC5 gene is mapped to human chromosome 17q21.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20 and 10% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Unit Definition

One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.