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Useful for the study of enzyme kinetics, screening inhibitors.

General description

Human HDAC1, GenBank Accession No. NM_004964, full length with C-terminal HIS-DDDDK tag (FLAG) and C-terminal His-tag, MW = 56 kDa, expressed in baculovirus expression system.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 130 mM NaCl, 0.05% Tween-20, 100 µg/ml FLAG peptide and 10% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Unit Definition

One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.