SAB5201915-100UG Display Image


Code: SAB5201915-100UG D2-231


Western blottingDilution 1:1000Tissue/Cell Type Used: HeLa cell lysateImmunofluorescenceDilution 1:100Tissue/Cell Type Used: HeLa


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£271.50 100UG


Western blottingDilution 1:1000Tissue/Cell Type Used: HeLa cell lysateImmunofluorescenceDilution 1:100Tissue/Cell Type Used: HeLa


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Immunogen type: Recombinant proteinImmunogen Species: HumanImmunogen Region: See Notes section/OtherImmunogen Description: Human recombinant ERp57 (Grp58) protein corresponding to amino acids 25 - 505 (his-tagged)

Other Notes

Additional Product Details:Immunogen region: Amino acids 25-505Lot Expiration/Retest Date: Expires 1 year from purchaseOther:Immunogen region: Amino acids 25-505

Purification method: Protein G purifiedProduct type: Primary antibodyAntibody structure: Whole AntibodyProtein ID: NP_005304.3

Physical form

50% glycerol, 0.09% Sodium Azide

PBS pH 7.4

Preparation Note

Derived from cell/tissue culture

Storage and Stability

Protect from light; avoid repeated freeze-thaw cycles. Store at -20 °C long-term, 2-8 °C short-term.

Target description

ERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs (1-5). It has quite a few diverse roles. It functions as an accessory oxidoreductase involved in disulfide bond formation. In the ER, ERp57 interacts with membrane bound calnexin and soluble calreticulin (lectin chaperones) via their praline rich P-domain arms. Lectin chaperones bind nascent non-native glycoproteins, and position ERp57 to act upon the immature or misfolded glycoproteins that possess mono-glycosylated side chains. ERp57 deletion impairs posttranslational phases of influenza hema-glutinin folding, and causes accelerated release of MHC-I molecules, resulting in the coupling of sub-optimal peptides and reduced expression and stability on the cell surface . ERp57 also contains two thioredoxin active-site sequences, CGHC and an estrogen-binding domain. ERp57 is induced by both estrogen and leuteinizing-hormone-releasing hormone in the hippocampus .

antibody formaffinity isolated antibody
antibody product typeprimary antibodies
biological sourcemouse
clone4F9, monoclonal
concentration1.0 mg/mL
conjugateAtto 390 conjugate
descriptiontarget molecular weight: 57 kDa
formbuffered aqueous solution
Gene Informationhuman ... PDIA3(2923)
packagingpkg of 100 µg
Protein ID accession no.NP_005304
shipped inwet ice
species reactivityhuman (Tested)
storage temp.−20°C
technique(s)western blot: 1:1000, immunofluorescence: 1:100
UniProt accession no.P30101
Est. Dispatch/Availability