SAB3700662-2MG Display Image

Anti-Rat IgG IgA IgM (H+L)-Fluorescein antibody produced in goat, IgG fraction of antiserum, lyophilized powder

Code: SAB3700662-2MG D2-231

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Immunofluorescence (1 paper)

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£211.00 2MG
List Price

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Immunofluorescence (1 paper)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Immunoglobulin G (IgG) is a widely expressed serum antibody. It belongs to the immunoglobulin family and consists of a gamma (?) heavy chain in the constant (C) region. The monomeric 150kDa structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weight of 50kDa and 25kDa, respectively. The primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named ?1, ?2, ?3, and ?4, respectively. Immunoglobulin M (IgM) exists as a pentamer in the serum and has a high molecular weight. Monomeric IgM has a molecular weight of 190kDa. The pentameric IgM consists of ten µ-heavy chains, ten light chains and a single J-chain which is linked by disulphide bonds. The µ -chain consists of five domains- VH, Cµ1, Cµ2, Cµ3 and Cµ4. Immunoglobulin A (IgA) is found as two subclasses, that is, IgA1 and IgA2. IgA1 has an extended hinge because of the insertion of a duplicated stretch of amino acids, whereas IgA2 lacks this. IgA exists as a monomer or dimer in the secreted form. Dimeric IgA has a molecular weight of 390kDa. This antibody can form multimers and its polymerization is essential for transport across epithelia. IgA is crucial as the first line of defence and has an important role in mucosal immunity.

Immunoglobulin G (IgG) is a widely expressed serum antibody. It belongs to the immunoglobulin family and consists of a gamma (?) heavy chain in the constant (C) region. The monomeric 150kDa structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weight of 50kDa and 25kDa, respectively. The primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named ?1, ?2, ?3, and ?4, respectively. Immunoglobulin M (IgM) exists as a pentamer in the serum and has a high molecular weight. Monomeric IgM has a molecular weight of 190kDa. The pentameric IgM consists of ten µ-heavy chains, ten light chains and a single J-chain which is linked by disulphide bonds. The µ -chain consists of five domains- VH, Cµ1, Cµ2, Cµ3 and Cµ4. Immunoglobulin A (IgA) is found as two subclasses, that is, IgA1 and IgA2. IgA1 has an extended hinge because of the insertion of a duplicated stretch of amino acids, whereas IgA2 lacks this. IgA exists as a monomer or dimer in the secreted form. Dimeric IgA has a molecular weight of 390kDa. This antibody can form multimers and its polymerization is essential for transport across epithelia. IgA is crucial as the first line of defence and has an important role in mucosal immunity.

Immunogen

Rat IgG, IgA and IgM whole molecules

Physical form

Supplied in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Physical properties

Antibody format: IgG

Reconstitution

Reconstitute with 1.0 mL deionized water (or equivalent).

Specificity

This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Fluorescein, Anti-Goat Serum, Rat IgG, Rat IgA and Rat IgM. This reagent is suitable for the detection of all Rat isotypes and chain combinations.

clonepolyclonal
antibody formaffinity isolated antibody
conjugatefluorescein conjugate
antibody product typesecondary antibodies
biological sourcegoat
application(s)indirect ELISA: suitable
application(s)direct immunofluorescence: suitable
application(s)indirect immunofluorescence: suitable
formlyophilized powder
Quality Level100
storage temp.2-8°C
shipped inwet ice
species reactivityrat