Features and BenefitsRapid - hot-start capability saves time, reduces primer-dimer formationSpecific and sensitive - for detection of a wide range of template concentrations, even from hard to obtain and low copy number samplesFast protocols - can be used with the new generation of fast cyclers without compromising performanceFlexible - suitable for block-based instruments where fluorescein is required to allow calculation of dynamic well factorsInstrument Compatibility Bio-Rad iCycler®, iQ®5, MyiQ™ (See product selection table) each of these instruments having the capacity to analyze the real-time PCR data with the passive reference signal either on or off, as well as several instruments that do not require the use of fluorescein.Description The SensiFAST SYBR & Fluorescein Kit uses a combination of the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, to ensure fast, highly-specific and ultra-sensitive real-time PCR (Fig. 1 & 2). SensiFAST SYBR & Fluorescein Kit has been optimized for use in SYBR Green-based real-time PCR. The SensiFAST SYBR & Fluorescein Kit contains premixed Fluorescein for optional use.Volume BIO-96001: 250 x 20µl Reactions: 2 x 1ml BIO-96005: 500 x 20µl Reactions: 5 x 1ml BIO-96020: 2000 x 20µl Reactions: 20 x 1ml Components 2x Reaction mix consisting of: Heat-activated DNA polymeraseUltra-pure dNTPsMgCl2FluoresceinSYBR Green IKit Size The kit size is based on a 20µl final reaction volume.Storage Conditions 12 months at -20°C. Avoid exposure of the SYBR® Green I to light.
This product has met the following criteria: