Application

Optimized and tested for isolation of histidine-tagged proteins. Chelating agents have been omitted to ensure full compatibility with IMAC applications.

Components

AEBSFBestatinE-64Pepstatin APhosphoramidon

Packaging

5 mL in glass bottle

1 mL in poly bottle

Preparation Note

This product is supplied as a clear solution in DMSO. One mL of solution is recommended for inhibition of protease activity in 100 mL of cell lysate from 20 g of E. coli cells or 10 g of baculovirus-infected cells.

Quantity

One mL is recommended for the inhibition of proteases extracted from 20 g of Escherichia coli or 10 g of baculovirus-infected Spodoptera frugiperda pupal ovary cells in a total volume of 100 ml.This protease inhibitor cocktail has been optimized and tested for histidine-tagged proteins

Specificity

Inhibits serine, cysteine, aspartic, and thermolysin-like proteases, and aminopeptidases.