Application

Optimized and tested for general use.

Biochem/physiol Actions

This mixture contains individual components, including AEBSF at 2 mM, Aprotinin at 0.3 µM, Bestatin at 116 µM, E-64 at 14 µM, Leupeptin at 1 µM and EDTA at 1 mM. Each component has specific inhibitory properties. AEBSF and Aprotinin act to inhibit serine proteases, including trypsin, chymotrypsin, and plasmin amongst others. Bestatin inhibits aminpeptidases. E-64 acts against cystein proteases. EDTA is an inhibitor of metalloproteases. Leupeptin acts against both serine and cystein proteases.

Caution

The lyophilized powder is stable for 4 years when stored at -20°C.

Components

AEBSFAprotininBestatinE-64EDTALeupeptin

Packaging

1 bottle in serum bottle

Preparation Note

This product is supplied as a lyophilized powder. One bottle prepares 100 mL of a cocktail solution.

Quantity

One bottle makes 100 mL of cocktail, which are sufficient to the inhibition of proteases present in approximately 20 gram of cell extract.Not all extracts contain the same levels of endogenous enzymes, and it may be necessary to adjust the volume of cocktail required.

Specificity

Inhibits serine, cysteine, and metallo-proteases.