Application

Nitrotetrazolium Blue chloride has been used to detect the generation of oxygen metabolites in cells. It has been used as a substrate for western blot detection. It has been used for the staining of histological tissue sections.

Biochem/physiol Actions

Nitrotetrazolium blue (NBT), in combination with BCIP (5-bromo-4-chloro-3-indolyl-phosphate), is required for the detection of labeled probes in western blots. It is part of the colorimetric detection assay. NBT and BCIP dyes detect alkaline phosphatase activity, thereby generating an insoluble purple dye (diformazan). The dye is visible at areas where the labeled probe has hybridized to its target.

Packaging

10×10, 50, 100, 250, 500 mg in glass insert

1, 5 g in glass bottle

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Substrates

Substrate for dehydrogenases and other oxidases.