Application

Nuclease from Staphylococcus aureus has been used in a study to assess coagulase and heat-resistant strains found in animals. It has also been used in a study to investigate the expression characteristic of two genes in S. aureus that encode two thermostable nucleases.

Biochem/physiol Actions

Hydrolyzes 5′-phosphodiester bonds of DNA.

Cleavage of DNA by Nuclease micrococcal is greater at the 5′ sides of A or T nucleotides than at G or C.

Other Notes

Note: One µmolar unit = approx. 85 A₂₆₀ units, where an A₂₆₀ unit is equivalent to a δA₂₆₀ of 1.0 in 30 min at pH 8.8 at 37 °C in a 3.55 mL reaction volume. Light path = 1 cm.

Packaging

50, 200, 500 units in serum bottle

Unit Definition

One unit will produce 1.0 µmole of acid soluble polynucleotides from native DNA per min at pH 8.8 at 37 °C, based on EM/260 = 10,000 for the mixed nucleotides.