N3254-25G Display Image

4-Nitrophenyl phosphate di(tris) salt, phosphatase substrate

Code: N3254-25G D2-231

Application

4-Nitrophenyl phosphate di(tris) salt has been used:as a substrate to quantify tartrate-resistant acid phosphatase (TRAP) activity in cell lysatesto dete...


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£447.00 25G
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Application

4-Nitrophenyl phosphate di(tris) salt has been used:as a substrate to quantify tartrate-resistant acid phosphatase (TRAP) activity in cell lysatesto determine phosphatase activity using native polyacrylamide gel electrophoresis (PAGE)for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA)

Biochem/physiol Actions

p-Nitrophenyl Phosphate (pNPP) is a preferred substrate for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures, because of its high sensitivity. This substrate produces a soluble yellow end-product and can be read spectrophotometrically at 405 nm.

assay≥97% (enzymatic)
formpowder
InChI keyXXAXKCWOTRABOW-UHFFFAOYSA-N
InChI1S/C6H6NO6P.2C4H11NO3/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2
Quality Level100
SMILES stringNC(CO)(CO)CO.NC(CO)(CO)CO.OP(O)(=O)Oc1ccc(cc1)[N+]([O-])=O
solubilitywater: 50 mg/mL, clear, colorless to light yellow
storage temp.−20°C
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Description
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N3254-5G
4-Nitrophenyl phosphate di(tris) salt, phosphatase substrate

Unit:5G
List Price: £115.00
Source:List Price
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N3254-100G
4-Nitrophenyl phosphate di(tris) salt, phosphatase substrate

Unit:100G
List Price: £1,070.00
Source:List Price
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N3254-1G
4-Nitrophenyl phosphate di(tris) salt, phosphatase substrate

Unit:1G
List Price: £40.60
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Cas Number68189-42-4
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