Application

Neomycin solution has been used:as a supplement of autoclaved acidified water for bone marrow transplantationfor the selection of stable clones as a component of artificial sea water (ASWO) medium to remove bacteria fromO. tauricultures

Biochem/physiol Actions

Neomycin is an aminoglycoside antibiotic, produced by Streptomyces containing a minimum of 85% neomycin B. It acts as a selection agent for prokaryotic cells transformed using the neo selectable marker gene. Neomycin trisulfate is used to study the cytototoxic side effects of antibiotics, platelet-derived growth factor responses in certain fibroblasts and extraction of nuclear phosphatidylinositol 4,5-bisphosphate-interacting proteins. This product is recommended for use in cell culture applications at 5 mL/L.Mode of action: This product acts by binding to the 30S and 50S subunits, causing miscoding and inhibiting initiation and elongation during protein synthesis. Neomycin also blocks voltage-sensitive Ca²⁺ channels without affecting the Na⁺/Ca²⁺ antiporter in neurons.Antimicrobial spectrum: Neomycin acts against both gram-positive and gram-negative bacteria

Caution

This solution is formulated to contain 10 mg/mL neomycin in 0.9% sodium chloride. It is stable at 37 °C for 5 days and should be otherwise stored at 2-8°C.

General description

Chemical structure: aminoglycoside