Application

IgG1, κ from murine myeloma has been used in:the in situ hybridization experiments with bulbar conjunctiva sectionsimmunohistochemistry of cattle and artery tissues

IgG1, Kappa from murine myeloma has been used in flow cytometric analysis and immunoprecipitation.

IgG1, κ from murine myeloma has been used:in immunofluorescence of leukocytes at a concentration of 50 μg/mlas a standard in enzyme linked immunosorbent assay (ELISA)as exchange antibody in centrifugal gel filtration

Biochem/physiol Actions

IgG antibody subtype is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

IgG1 has a molecular weight of 146 kDa and is an abundantly expressed IgG subclass. IgG1 deficiencies contribute to overall decrease in total IgG levels resulting in hypogammaglobulinemia. IgG1 promotes bacterial phagocytosis and mediates vaccine-induced protection from infection. The ratio of IgG2a to IgG1 is crucial for the immune response against Leishmania tropica infection. It has high binding affinity towards the Fcγ receptors (FcγR). High levels of IgG1-κ antibodies is associated with the pathology of glomerulonephritis.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Physical form

Solution in 0.02 M Tris buffered saline, pH 8.0, containing 0.02% sodium azide

Specificity

Specificity is determined by mouse monoclonal isotyping strips. The purified immunoglobulin preparation is non-reactive with anti mouse IgA, IgM, IgG2a, IgG2b or IgG3

Storage and Stability

Store at −20 °C. The product may be stored frozen in working aliquots at −20 °C. Repeated freezing and thawing is not recommended.