M9163-500ML Display Image

Medium 199, 10 x, With Hanks salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Code: M9163-500ML D2-231

General description

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the c...


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£106.00 500ML

General description

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Reconstitution

Supplement with 0.1 g/L L-glutamine, 0.35 g/L sodium bicarbonate at 1×.

componentsL-glutamine: noHEPES: nosodium pyruvate: noNaHCO3: nophenol red: yesHanks’; salts (2% CO2): yes
concentration10 ×
formliquid
impuritiesendotoxin, tested
Quality Level400
shipped inambient
sterilitysterile-filtered
storage temp.2-8°C
technique(s)cell culture | mammalian: suitable
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Pack500ML
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