Application

Malonate broth has been used to evaluate the utilization of malonate by Enterobacter sakazakii. It has also been used to differentiate between Cronobacter sakazakii and Cronobacter malonaticus isolates.

Components

Components (g/L)Ammonium sulfate, 2.00Dipotassium phosphate, 0.60Monopotassium phosphate, 0.40Sodium chloride, 2.00Sodium malonate, 3.00Bromo thymol blue, 0.025

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

General description

Malonate broth is recommended for the differentiation of Enterobacter and Escherichia.

Legal Information

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

Preparation Note

Suspend 8 g of Malonate Broth in 1000 ml of distilled water. Dispense and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Avoid the addition of carbon and nitrogen from other sources.