Application

Anti-phospho-Lck (pTyr192) antibody can be used in western blotting (diluted 1:1000) using full length recombinant human Lck protein.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Lck, also referred to as T cell-specific protein tyrosine kinase, is a 56kD protein that belongs to Src family of non-receptor tyrosine protein kinases and is expressed mainly in T cells. It has a pivotal role in the initiation of pre-TCR and T-cell antigen receptor (TCR)-linked signal transduction pathways. Anti-phospho-Lck (pTyr192) antibody reacts specifically with Lck (pTyr192) in humans.

Immunogen

synthetic phosphopeptide derived from the region of Lck that is phosphorylated on tyrosine 192. Human and mouse have 100% homology.

Physical form

Solution in Dulbecco′s phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.3 (+/- 0.1), 50% glycerol, with 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide.

Preparation Note

The antibody is preadsorbed to remove any reactivity towards a non-phosphorylated Lck.