L4130-100MG Display Image

Lipopolysaccharides from Escherichia coli 0111:B4, purified by trichloroacetic acid extraction

Code: L4130-100MG D2-231

Application

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immu...

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£153.00 100MG
List Price

Application

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Biochem/physiol Actions

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

General description

This product is TCA extracted from E. coli serotype O111:B4. The source strain is from a private collection. This LPS serotype has been used to stimulate B-cells and induce NOS in human hepatocytes.

Packaging

100, 500 mg in poly bottle

Preparation Note

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Quality Level200
storage temp.2-8C
biological sourceEscherichia coli (O111:B4)
shipped inambient
purified bytrichloroacetic acid extraction
formlyophilized powder
impurities1-10% Protein (Lowry)

Code
Description
Unit Size
List Price
Qty
L4130-500MG
Unit:500MG
List Price: £605.00
Source:List Price
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