L3129-25MG Display Image

Lipopolysaccharides from Escherichia coli 0127:B8, purified by phenol extraction

Code: L3129-25MG D2-231

Application

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immu...

read more

£61.90 25MG
List Price

Application

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Biochem/physiol Actions

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

General description

This product is phenol extracted from E. coli serotype O127:B8. The source strain is ATCC 12740. This LPS serotype has been used in the study of septic shock and to induce NOS in murine macrophages and PAF synthesis in rat glomerular masangial cells.

Packaging

25, 100 mg in glass bottle

10 mg in poly bottle

Preparation Note

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

biological sourceEscherichia coli (O127:B8)
Quality Level200
purified byphenol extraction
storage temp.2-8C
impurities3% Protein (Lowry)
shipped inambient
formlyophilized powder

Code
Description
Unit Size
List Price
Qty
L3129-100MG
Unit:100MG
List Price: £134.00
Source:List Price
ADD
L3129-10MG
Unit:10MG
List Price: £29.10
Source:List Price
ADD