L2380-1MG Display Image

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia), lyophilized powder

Code: L2380-1MG D2-231

Analysis Note

Agglutination activity is expressed in µg/mL and is determined from serial dilutions of a 1 mg/mL solution using phosphate buffered saline, pH 6.8, contain...

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£57.80 1MG
List Price

Analysis Note

Agglutination activity is expressed in µg/mL and is determined from serial dilutions of a 1 mg/mL solution using phosphate buffered saline, pH 6.8, containing, for each lectin, calcium, magnesium, and manganese at different concentrations. This activity is the lowest concentration to agglutinate a 2% suspension of appropriate erythrocytes after 1 hr incubation at 25 °C.

Biochem/physiol Actions

BS-I has a major affinity for terminal a-D-galactosyl residues with a secondary affinity for terminal N-acetyl-a-D-galactosaminyl residues.

Other Notes

BS-I is a tetrameric lectin consisting of two types of subunits designated A and B. There are five BS-I isolectins with different subunit composition: BSI-B4, BSI-AB3, BSI-A2B2, BSI-A3B and BSI-A4. BSI-B4 is blood group B specific and has an exclusive affinity for terminal α-D-galactosyl residues, whereas BSI-A4 has blood group A specificity and has a major affinity for terminal N-acetyl-α-D-galactosaminyl residues.

Packaging

1, 5 mg in glass bottle

Preparation Note

Purified by affinity chromatography using the method of Hayes, C. E., and Goldstein, I. J., J. Biol. Chem., 249, 1904 (1974).

impuritiessalt, free
Quality Level200
storage temp.2-8°C
potency≤20 µg per mL agglutination activity (using human blood group B erythrocytes)
compositionProtein, ≥90% biuret
formlyophilized powder

Code
Description
Unit Size
List Price
Qty
L2380-5MG
Unit:5MG
List Price: £194.00
Source:List Price
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