L2018-5MG Display Image

Lipopolysaccharides from Escherichia coli K-235, purified by gel-filtration chromatography

Code: L2018-5MG D2-231

Biochem/physiol Actions

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contri...

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£146.00 5MG
List Price

Biochem/physiol Actions

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

General description

This product is extracted from E. coli serotype K235 and purified by gel filtration. The source strain is ATCC 13027.

Packaging

5, 25 mg in glass bottle

Preparation Note

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

biological sourceEscherichia coli (K-235)
Quality Level200
storage temp.2-8C
purified bygel-filtration chromatography
shipped inambient
formlyophilized powder
impurities1% Protein