Agglutination activity is expressed in µg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of neuraminidase treated human blood group O erythrocytes after 1 hr incubation at 25 °C.
Lectin from Arachis hypogaea (peanut) has been used:• to detect Thomson-Friedenreich antigen• as a component in PBS to study its effects on cell viability• to study its ability to block the binding site
PNA does not agglutinate normal human erythrocytes, but strongly agglutinates neuraminidase treated erythrocytes. PNA has potent anti-T activity similar to the anti-T antibody in human sera. The lectin can be used to distinguish between human lymphocyte subsets.
Lectin from Arachis hypogaea (peanut) possess specificity towards ß -D-Gal(1-3)-D-galNAc residue of Thomson - Friedenreich antigen. It is the most widely used diagnostic and prognostic marker for many squamous cell carcinomas.
Lectin from Arachis hypogaea (peanut) is a homotetrameric protein of 110 kDa.
5, 10, 25 mg in glass bottle