L0770-2MG Display Image

Lectin from Pisum sativum (pea), FITC conjugate, lyophilized powder

Code: L0770-2MG D2-231

Analysis Note

Agglutination activity is expressed in µg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This acti...

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£150.00 2MG
List Price

Analysis Note

Agglutination activity is expressed in µg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

Application

Lectin from Pisum sativum (pea) has been used to determine acrosomal status and viability of spermatozoa and to assess sperm acrosome reaction (AR) patterns

Biochem/physiol Actions

PSA is not blood group specific, but has an affinity for terminal α-D-mannosyl residues. PSA is a mitogen similar to concanavalin A.

Lectin from Pisum sativum (pea) possess anti-hepatoma effect and mitogenicity to lymphocytes, like concanavalin A (Con A).

General description

Lectin from Pisum sativum (pea) is a tetrameric glycoprotein of about 48 kDa. It is isolated from peas. Pisum sativum agglutinin is specific for a-D-glucose and a-D-mannose.

Packaging

2 mg in serum bottle

Preparation Note

Conjugates are prepared from affinity purified lectin.

impuritiessalt, free
Quality Level200
compositionProtein, 95% biuret
extent of labeling2-4 mol FITC per mol protein (Lowry).
conjugateFITC conjugate
formlyophilized powder
potency20 g per mL agglutination activity
storage temp.20C