Application

Technical grade IgG may be used as an economical alternative to the reagent grade immunoglobulins in applications where high purity is not required. IgG from mouse serum was used as negative control in immunohistochemistry at a concentration of 2 μg/ml. It was used as irrelevant control in chromatin immunoprecipitaion assay (10 μg).

Biochem/physiol Actions

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections IgG from mouse serum specifically recognizes IgG from mouse. Mouse IgG is purified from normal mouse serum by fractionation.

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. A immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3.

Packaging

10 mg in glass bottle

5 mg in glass insert

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.2, containing 15 mM sodium azide