Analysis Note

Immunoelectrophoresis (IEP) is used to establish the identity of the Rabbit IgG. . A single major arc of precipitation in the gamma region is observed versus anti-rabbit whole serum and anti-rabbit IgG. Minor gamma arcs may be present versus anti-rabbit whole serum

Application

IgG from rabbit serum has been used:as a reference, standard or blocking agent in many immunoassays including dot blots, ELISA, Westerns, IEP, and immunodiffusion assaysin arthus reactionin chromatin immunoprecipitation

Biochem/physiol Actions

Immunoglobulin G (IgG) regulates the immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. IgG1 regulates complement fixation in mice.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Packaging

10, 50 mg in glass bottle

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.2, containing 15 mM sodium azide

Preparation Note

Rabbit IgG is purified via fractionation from pooled normal rabbit serum