Application

Ionomycin calcium salt from Streptomyces conglobatus has been used as a media supplement to culture:peripheral blood mononuclear cells (PBMCs) for immunologic assessmentoocytes for parthenogenetic activationT cell subsets for the detection of intracellular cytokines, carboxyfluorescein diacetate succinimidyl ester (CFSE) proliferation assay and antigen-specific suppressive function assay

Biochem/physiol Actions

Ionomycin is an ionophore, widely used for validation and calibration of calcium analysis.

Ca²⁺ ionophore that is more effective than A23187 as a mobile ion carrier for Ca²⁺; non-fluorescent; used to study Ca²⁺ transport across biological membranes; induces apoptotic degeneration of embryonic cortical neurons.

Packaging

1, 5 mg in glass bottle

Preparation Note

Store ionomycin in powder form desiccated and protected from light at 2-8 °C. Under these conditions the product is stable for 3 years. Store stock solutions in ethanol or DMSO at -20 °C, protected from light. Under these conditions the stock solutions are stable for several months. For short term use (up to 6 weeks) the stock solutions maybe stored at 2-8 °C protected from light.