Application

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies^® protocols and other useful information.

Biochem/physiol Actions

The gene TMEM204 (transmembrane protein 204) encodes a tetraspan adherens junction protein that is hypoxically regulated. It may be involved in the regulation of cell adhesion, paracellular permeability and angiogenesis. Over expression of this protein leads to reduced adhesion between cells and variation of its junctional barrier function.

Features and Benefits

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.Every Prestige Antibody is tested in the following ways: IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. Protein array of 364 human recombinant protein fragments.

General description

The gene TMEM204 (transmembrane protein 204) encodes a four transmembrane spanning protein that is commonly called CLP24 (claudin-like protein of 24kDa). It is found to be homologous to myelin protein 22/epithelial membrane protein 1/claudin family of cell junction proteins. These homologous proteins modulate paracellular permeability. The encoded protein contains a protein-protein interaction domain at the C-terminus. It is abundantly expressed in lung, heart, kidney and placental tissues. It localizes to cell-cell junctions and is found to exist along with the β-catenin adherens junction-associated protein. It does not co-exist with tight junctions.

Immunogen

Transmembrane protein 204 recombinant protein epitope signature tag (PrEST)

Legal Information

Prestige Antibodies is a registered trademark of Sigma-Aldrich Co. LLC

Linkage

Corresponding Antigen APREST72616.

Physical form

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide