Application

RNAse HEPES has been used:To supplement Dulbecco’;smodified Eagle’;smedium for maintenance of cell line and RPMI medium to wash rat isletsTo recover purified ribonucleotideAs a component of HEPES/KOH buffer and adenylation buffer for small RNA isolation and sequencingAs a component in buffers used for nuclear extract preparation and also to supplement RPMI-1640 medium for maintenance of islets

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.