Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Application

Protein G Mag Sepharose^® are magnetic beads designed to simplify enrichment of target Proteins by immunoprecipitation or pull-down applications. The magnetic beads are based on Sepharose^® with Protein G as ligand. Protein G has high affinity for the Fc region of IgG antibodies from a broad variety of species. There are two protocols available for Protein enrichment: the cross-link protocol and the classic protocol. The protocols are simple with elution conditions optimized for both electrophoresis and MS analysis. Protein G Mag Sepharose^® combines well-proven enrichment methods with the magnetic beads platform, which has excellent properties for small-scale sample preparation. Together with MagRack 6, a separation tool for six microcentrifuge tubes, up to six samples can be processed in parallel.

Features and Benefits

•Visible and dense Sepharose^® based magnetic beads with Protein A ligand, easy to spot and collect bound target Protein•Non-adherent beads eliminate smearing effects and aggregate formation. Can be used without detergents•Simple capture of target Protein in small or large sample volumes (Low microliter to high milliliter scale)•Optimized capacity for enrichment/immunoprecipitation of target Proteins - only Low amounts of antibody needed•Flexible protocols with elution conditions optimized for both electrophoresis and MS analysis

General description

Protein G Mag Sepharose^® are magnetic beads designed to simplify enrichment of target proteins by immunoprecipitation applications. Based on Sepharose^® with Protein G as ligand. Protein G has high affinity for the Fc region of IgG antibodies.

Legal Information

Sepharose is a registered trademark of Cytiva