Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Application

The small column volume of 4.7 mL and bed height of 10 cm make HiScreen^™ columns excellent tools for method optimization, parameter screening, robustness testing, and convenient scale-up. Process fluid velocities can be applied, since the 10 cm bed height gives enough residence time and the results can then serve as basis for linear process scale-up. If necessary, two columns can easily be connected in series to give a bed height of 20 cm. Media prepacked in HiScreen^™ HIC columns are also available in other formats and as bulk packs, which makes it possible to use the same media for development work, pilot studies, and routine production.

Features and Benefits

Butyl Sepharose^® High Performance is an aliphatic HIC medium, designed for intermediate and polishing step purification steps when high resolution has priority. Prepacked, ready-to-use columns for convenient process development optimization. 10 cm bed height of HiScreen^™ columns is designed to allow method optimization and parameter screening. Easily connected in series to achieve 20 cm bed height. Small bed volume gives fast results and minimal sample/buffer consumption. Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity.

General description

HiScreen^™ Phenyl HP columns are prepacked with Phenyl Sepharose^® High Performance hydrophobic interaction chromatography (HIC) media. The columns are an excellent choice for method optimization and parameter screening.

Legal Information

Sepharose is a registered trademark of Cytiva

HiScreen is a trademark of Cytiva

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Store at 4 to 30 °C (20% Ethanol or 0.01 M NaOH)