Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Features and Benefits
Optimized for convenient scale-up purification of histidine-tagged proteins. Compatible with a wide range of reducing agents, detergents, denaturants, and other additives. Negligible leakage of Ni2+. High binding capacity, approx. 40 mg/mL medium. Convenient and time-saving 20 mL prepacked HiPrep™ format.
General description
HisPrep™ FF 16/10 is a ready-to-use column, prepacked with precharged Ni Sepharose® 6 Fast Flow. The column is an excellent choice for preparative, scale-up purification of histidine-tagged recombinant proteins.HisPrep™ FF 16/10 columns are prepacked with Ni Sepharose® 6 Fast Flow for scale-up purification of histidine-tagged proteins. Ni Sepharose® 6 Fast Flow consists of 90 ?m beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni2+ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni2+ ions ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.
Legal Information
HiPrep is a trademark of Cytiva
HisPrep is a trademark of Cytiva
Sepharose is a registered trademark of Cytiva
Storage and Stability
Store at 4 to 30 °C (20% Ethanol)
This product has met the following criteria: