Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Application

The kit can be used with both small (e.g., plasmids and M13) and large (fosmids and bacterial artificial chromosomes [BACs]) circular templates. The starting template for the reaction can be purified DNA, glycerol stock, liquid culture, or colonies. The reaction takes less than 20 min to prepare and after an overnight incubation at 10°C, it is ready to be used directly in sequencing applications with the sequencing chemistry of choice. A ten microliter (10 µL) reaction typically yields 1 µg of DNA in a standard overnight reaction.

Features and Benefits

For the amplification of difficult templates for successful DNA sequencing. Solve the most common sequencing problems such as repeats, sequencing stops, and compressions with a single kit. Improve difficult template sequencing success with up to 820 bp Phred 20 read length. Cultureless preparation from bacterial colonies, glycerol stock or purified DNA; saves time and reagents. Easy-to-use kit requires only 20 min hands-on time. Amplified DNA can be directly used in sequencing reactions. illustra^™ TempliPhi^™ Sequence Resolver Kit produces exceptional sequencing results from difficult templates such as those with high GC content and secondary structures. The kit resolves most of the sequencing stops or gaps that routinely elude other commonly used finishing methods.

General description

The illustra^™ TempliPhi^™ Sequence Resolver Kit uses the highly processive, strand-displacing Phi29 DNA polymerase and modified nucleotides to amplify circular templates for subsequent sequencing.

Legal Information

TempliPhiOn packaging and insert for kits:For use only as licensed by Qiagen GmbH. and Cytiva. The Phi 29 DNA polymerase may not be re-sold or used except in conjunction with the other components of this kit. See US patent numbers 5,854,033, 6,124,120, 6,143,495, 6,323,009, 5,198,543, 5,576,204, and their equivalents in other countries.On container:Subject to proprietary rights of Cytiva. May not be re-sold or used except with the other components of this kit. See US patent numbers 5,576,204, and their equivalents in other countries.

TempliPhi is a trademark of Cytiva

illustra is a trademark of Cytiva