Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Application

Protein A Sepharose^® HP has high affinity for the Fc region of IgG from a variety of species. The two options in the Protein enrichment protocol allow the attached antigen (Protein of interest) to be eluted separately (cross-link protocol) or together with the antibody (classic protocol). There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.

Features and Benefits

For simple, small-scale antibody purification or enrichment of specific Proteins in prepacked, single-use spin columns that are ready to use. Classic and cross-link protocols provide flexibility. Elution conditions formatted for both electrophoresis and LC-MS analysis workflows. Fast binding kinetics and high capacity provide high yield. Prepacked with Protein A Sepharose^® High Performance for coupling of antibodies of IgG subclasses. Easy scale-up with HiTrap^® Protein A HP prepacked columns.

General description

protein A HP SpinTrap^™ are designed for the enrichment of proteins of interest, immunoprecipitation and the purification of monoclonal and polyclonal antibodies from cell lysates and biological fluids. Contain protein A Sepharose^® HP.

Legal Information

Sepharose is a registered trademark of Cytiva

SpinTrap is a trademark of Cytiva

HiTrap is a registered trademark of Cytiva