Analysis Note

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Application

GST, see the section for licensing information in our terms and conditions, tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap^™ 4B. GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves Protein antigenicity and function. If desired, cleavage of the Protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods. The medium, Glutathione Sepharose^® 4B, is also available as lab packages and is a good choice for scale-up. The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA^® design or FPLC^™ System.

Features and Benefits

• High binding capacity.• Mild elution conditions preserving Protein antigenicity and function.• Easy one-step purifications of Glutathione S-Transferase (GST) tagged Proteins resulting in high purity.• GSTrap^™ 4B are designed to be used with a syringe, pump, or chromatography system, such as ÄKTA^® design.

General description

GSTrap^™ 4B columns are prepacked Glutathione Sepharose^® 4B columns for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins.

Legal Information

ÄKTA is a registered trademark of GE Healthcare

Sepharose is a registered trademark of GE Healthcare

GSTrap is a trademark of GE Healthcare

FPLC is a trademark of GE Healthcare

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)