Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Features and Benefits

• For simple, small-scale purification of histidine-tagged* proteins and rapid expression screening.• Ni Sepharose^® High Performance in prepacked spin columns.• Yields up to 750 µg pure protein per column.• Allows direct purification of both clarified and unclarified cell lysates.

General description

His SpinTrap^™ is a prepacked, single-use spin column for purifying histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC)* See the section for licensing information in our terms and conditions.Purification of unclarified samples on His SpinTrap^™ columns minimizes loss of target protein caused by manual operations such as sample precentrifugation, transfer to centrifugation tubes, and collecting supernatant. In addition, loading unclarified sample directly to His SpinTrap^™ columns reduces sample preparation time, which minimizes degradation and oxidation of sensitive target proteins.His SpinTrap^™ columns are used together with a standard microcentrifuge and one purification run takes approximately 10 min. For optimal performance, use His SpinTrap^™ together with buffers prepared using His Buffer Kit.The columns are prepacked with Ni Sepharose^® High Performance, which is precharged with nickel ions for capture of histidine-tagged proteins. Leakage of nickel ions from the Ni Sepharose^® High Performance medium is negligible.

Legal Information

SpinTrap is a trademark of GE Healthcare

Sepharose is a registered trademark of GE Healthcare

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Store at 4 to 30 °C (0.15% Kathon CG)