His MultiTrap™ FF and His MultiTrap™ HP are prepacked 96-well filter plates for high-throughput screening of histidine-tagged* Proteins from clarified or unclarified lysates. Purification is performed by immobilized metal ion affinity chromatography (IMAC). The plates are prepacked with Ni Sepharose® 6 Fast FLow (His MultiTrap™ FF) or Ni Sepharose® High Performance (His MultiTrap™ HP). A protocol for His MultiTrap™ is available in His Buffer Kit. The kit is available with ready-made buffer solutions, for increased convenience and for fast buffer preparation.Loading unclarified samples directly to His MultiTrap™ FF and His MultiTrap™ HP simplifies and shortens the overall time for expression screening, which minimizes degradation and oxidation of sensitive target Proteins. His MultiTrap™ FF and His MultiTrap™ HP prepacked filter plates are manufactured with high reproducibility with regards to the well volume and the volume of medium to give consistent results.
Features and Benefits
• Prepacked multiwell filter plates for high reproducibility in expression screening and small-scale purification of histidine-tagged* Proteins.• Increased convenience and consistency with prepacked 96-well plates.• No filtration needed - load unclarified sample directly, increase reproducibility, and save time.• Use His MultiTrap™ 96-well filter plates with robotic systems or manually with centrifugation or vacuum.• Easy scale-up to HisTrap™ FF, HisPrep™ FF 16/10 or HisTrap™ HP prepacked columns. High chemical stability and high binding capacity up to 1 mg of histidine-tagged Protein per well.
His MultiTrap™ FF and His MultiTrap™ HP are prepacked disposable 96-well plates for reproducible high throughput parallel purification of histidine-tagged recombinant proteins by Immobilized Metal ion Affinity Chromatography (IMAC).
MultiTrap is a trademark of GE Healthcare
Sepharose is a registered trademark of GE Healthcare
HisTrap is a trademark of GE Healthcare
HisPrep is a trademark of GE Healthcare
Storage and Stability
Store at 4 to 30 °C (20% Ethanol)