Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Application

Used for site-specific separation of the GST tag from proteins expressed using pGEX-T vectors

Features and Benefits

• Enables the site-specific cleavage of fusion proteins containing an accessible thrombin recognition sequence• Purified from bovine plasma; functionally free of other clotting factors, plasminogen, and plasmin• Can be used either following affinity purification or while fusion proteins are bound to Glutathione Sepharose^® 4B, Glutathione Sepharose^® High Performance, or Glutathione Sepharose^® 4 Fast Flow

General description

Thrombin is a protease used to digest fusion proteins prepared from pGEX vectors recognition sequence for thrombin (pGEX-1lT, pGEX-2T, pGEX-2TK, pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3). Purified from bovine plasma.Thrombin is a protease used to digest fusion proteins prepared from pGEX vectors (see pGEX Vectors (GST Gene Fusion System)) containing the recognition sequence for thrombin (pGEX-1lT, pGEX-2T, pGEX-2TK, pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3).

Legal Information

Sepharose is a registered trademark of GE Healthcare