Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Application

For PCR and other procedures requiring DNA polymerase activity at elevated temperatures

Features and Benefits

Highly purified thermostable DNA polymerase for PCR. Highly purified recombinant enzyme. Excellent batch-to-batch reproducibility. Each lot is tested to be free of nuclease contamination.

General description

Taq DNA Polymerase (cloned) is the recombinant form of the native enzyme from Thermus aquaticus expressed in E. coli. Like native Taq, it polymerizes DNA from a primer annealed to a DNA template in the presence of deoxyribonucleotide triphosphates. Because Taq DNA Polymerase (cloned) is a recombinant protein, it is exceptionally pure and provides excellent batch-to-batch reproducibility. It has an optimum temperature of 75°C and can survive repeated incubations at > 95°C

Legal Information

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser’;s own internal research. No other patent rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Forest City, California 94404, USA.

Packaging

100 mM Tris-HCl, pH 9.0, 15 mM MgCl₂ and 500 mM KCl. Also supplied with 25 mM MgCl₂ solution