Application

Guanidine thiocyanate has been used:for the lysis of colorectal adenocarcinoma cell line prior to RNA isolationas a PCR inhibitor in triplex mitochondrial DNA (mtDNA) qPCR assayfor DNA extraction from cerebrospinal fluid (CSF)

Chaotropic agent and strong denaturant; solubilizes cells.

Biochem/physiol Actions

Guanidine thiocyanate is a denaturing agent and is used routinely in RNA isolation. It is used as a storage buffer for whole blood samples. Guanidine thiocyanate inactivates nucleases and is ideal for storing and freezing fecal samples for DNA studies. It is used in combination with phenol−chloroform in RNA extraction.

Packaging

100, 250, 500 g in poly bottle