Application

Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

New Technical Article Comparing Performance of Different EnzymesLearn more about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

Effective in the hydrolysis of steroid glucuronides.

Packaging

25000, 500000, 2000000 units in glass bottle

Quality

Contains buffer salts and stabilizer.

Unit Definition

One Sigma or modified Fishman unit will liberate 1.0 µg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at the pH 6.8 (30 min assay).