Application

The antibody recognizes the FLAG epitope located on FLAG-tagged fusion proteins at the N-terminus or C-terminus, applying dot blot, immunoblotting, immunoprecipitation and immunocytochemistry assays. Browse additional application references in our FLAG® Literature portal."

Biochem/physiol Actions

FLAG epitope plays a crucial role in immunoaffinity purification of fusion proteins.

General description

The rabbit Anti-FLAG polyclonal affinity antibody ANTI-FLAG recognizes the FLAG epitope located on FLAG fusion proteins. This antibody reacts with N-terminal, N-terminal-Met, and C-terminal FLAG fusion proteins.

The FLAG epitope, is an eight amino acid protein with an enterokinase-cleavage site.

Immunogen

FLAG; peptide sequence DYKDDDDK

Legal Information

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Preparation Note

Purified by affinity chromatography on a column bearing the immunizing peptide.