Application

Anti-Ferritin, Human antibody is suitable for use in Ouchterlony double diffusion (1:16 using human spleen ferritin), and immunoelectrophoresis. The antibody may also be used in western blot (1:1000 using frozen liver homogenates) and immunoprecipitation (using 300 μg of cell lysate incubated with 6 mul of anti-ferritin for 1h at 4 ° C).

Anti-Ferritin, Human antibody produced in rabbit has been used in:immunohistochemistrywestern blottingimmunocytochemical techniques

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Western Blotting (1 paper)

Biochem/physiol Actions

Ferritin is an iron-storage protein that regulates iron-homeostasis in cells. While mitochondrial ferritin is a homopolymer, the cytosolic form consists of heteropolymeric L- and H-chains. Genetic mutations in ferritin L-chains have been associated with neurodegenerative disorders, whereas altered levels of ferritin have been linked to hemophagocytic lymphohistiocytosis and adult-onset Still′s disease . In immunoelectrophoresis, anti-ferritin, human antibody is specific for ferritin when used against human spleen extract and purified ferritin. No reactivity is observed against normal human serum.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Ferritin, an iron-storage protein is usually present in the liver and spleen in mammals. Iron andferritinare distributed relatively same in the eye. Ferritin is made up of heavy and light chains.

Immunogen

Ferritin isolated from normal human spleen

Preparation Note

The antiserum has been treated to remove lipoproteins.