Application

Fibronectin from bovine plasma is used for the following applications:Used for coating the glass coverslips for the culture of decidual stromal cells (DEC)Cell movement assays (culture plates were coated with fibronectin)Cell spreading and immunofluorescence Protein specificityIntegration of sensor films into microfluidic chipsHistological and Immunohistochemical (IHC) analysesUsed in primary cell cultures from patients with lung carcinoma

Biochem/physiol Actions

Fibronectin is aregulator of protein scaffolding. It coordinates cellular processes like platelet functions, wound-healing, adhesion, migration and aggregation.

Caution

As supplied, this product should be stored at -20°C and will remain active for at least 2 years.

General description

Fibronectin is a glycoprotein with multiple domains. It comprises a N-terminal, central binding domain and a heparin-binding domain. Bovine plasma fibronectin has polypeptide chains with disulfide linkage and corresponds to a molecular weight of 220 and 215 kDa.

Fibronectin (FN) is a multifunctional glycoprotein, the gene of which is localized to human chromosome 2q34-36. This gene spans 75kb and contains 50 exons. It exists as two major isoforms, one soluble and one insoluble form. The former is present in plasma, whereas the insoluble form resides in tissues and extracellular matrix (ECM) of cartilage. There are around 20 different forms of FN due to alternative splicing of a variable region and two type III exons called Extra Domains A and B.