Application

Anti-Mouse IgG (Fc specific) F(ab′)2 fragment-FITC antibody produced in goat has been used in : flow cytometry for labelling of human peripheral blood lymphocytes (dilution of 1:100) and label human polymorphonuclear cells (PMNs) (dilution of 1:50).immunocytochemistry using human embryonic stem cellsimmunohistochemistry at a working dilution of 1:160.

Goat polyclonal anti-Mouse IgG (Fc specific) F(ab′)2 fragment-FITC antibody may be used as a reagent in flow cytometry, immunohistology and immunocytology offering sensitive and specific activity to mouse IgG and no cross reactivity with human, horse or bovine immunoglobulins. Immunohistology: A minimum dilution of 1:160 is determined by indirect immunofluorescence on formalinfixed, and paraffin-embedded human tonsil using mouse monoclonal anti-human IgG as the primary antibody.

Biochem/physiol Actions

immunoglobulin G (IgG) antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. IgG antibody participates in complement fixation and opsonization.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection. Anti-mouse IgG is conjugated to Fluorescein Isothiocyanate (FITC), Isomer I. The F(ab′)₂ antibodies isolated from goat antiserum by affinity chromatography, react specifically with mouse IgG Fc; does not bind other mouse Igs. No cross reaction is observed with mouse Fab fragment. The antibody shows minimal cross reaction with human, horse and bovine proteins on tissue or cell preparations.

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids.Fluorescein isothiocyanate (FITC) is a fluorescein derivative (fluorochrome) used to tag antibodies, including secondary antibodies, for use in fluorescence-based assays and procedures. FITC excites at 495 nm and emits at 521 nm.

Immunogen

Purified Fc fragments of mouse IgG

Other Notes

Antibody adsorbed with bovine, equine and human serum proteins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Preparation Note

Adsorbed to reduce background with bovine, horse or human samples.Useful when trying to avoid background staining due to the presence of Fc receptors.

Specificity

Goat polyclonal anti-Mouse IgG (Fc specific) F(ab′)2 fragment−FITC antibody isolated from goat antiserum by affinity chromatography reacts specifically with mouse Fc fragment. No cross reaction is observed with mouse Fab fragment. The antibodies are adsorbed to assure minimal cross reaction with human, horse and bovine proteins on tissue or cell preparations.